Literature summary for 2.8.2.1 extracted from

Beckmann, J.; Chodavarapu, S.; Doyle, B.
Evaluation of a conserved tryptophanyl residue in donor substrate binding and catalysis by a phenol sulfotransferase (SULT1A1) (2020), Arch. Biochem. Biophys., 695, 108621 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
homology modeling of the active site. Two phenylalanyl and one tyrosinyl group help create a hydrophobic pocket for phenolic acceptor substrates. Two arginyl and one lysyl residues, plus other groups, aid in the binding of 3'-phosphoadenosine-5'-phospho(sulfate) via electrostatic forces and H-bonding. The conserved histidine serves as the general base to initiate phenoxide anion stabilization and attack on the 3'-phosphoadenosine-5'-phosphosulfate sulfuryl group	Bos taurus

Crystallization (Comment)

Organism

homology modeling of the active site. Two phenylalanyl and one tyrosinyl group help create a hydrophobic pocket for phenolic acceptor substrates. Two arginyl and one lysyl residues, plus other groups, aid in the binding of 3'-phosphoadenosine-5'-phospho(sulfate) via electrostatic forces and H-bonding. The conserved histidine serves as the general base to initiate phenoxide anion stabilization and attack on the 3'-phosphoadenosine-5'-phosphosulfate sulfuryl group

Bos taurus

Protein Variants

Protein Variants	Comment	Organism
W53A	mutation decreases thermal stabilizations by 3'-phosphoadenosine-5'-phosphate plus pentachlorophenol	Bos taurus
W53L	mutation decreases thermal stabilizations by 3'-phosphoadenosine-5'-phosphate plus pentachlorophenol	Bos taurus
W53Y	mutation decreases thermal stabilizations by 3'-phosphoadenosine-5'-phosphate plus pentachlorophenol	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.000526	-	7-hydroxycoumarin	wild-type, pH 7.4, temperature not specified in the publication	Bos taurus
0.000815	-	3'-phosphoadenylyl sulfate	wild-type, pH 7.4, temperature not specified in the publication	Bos taurus
0.001394	-	3'-phosphoadenylyl sulfate	mutant W53Y, pH 7.4, temperature not specified in the publication	Bos taurus
0.00187	-	7-hydroxycoumarin	mutant W53A, pH 7.4, temperature not specified in the publication	Bos taurus
0.00208	-	7-hydroxycoumarin	mutant W53Y, pH 7.4, temperature not specified in the publication	Bos taurus
0.003	-	7-hydroxycoumarin	mutant W53L, pH 7.4, temperature not specified in the publication	Bos taurus
0.00519	-	3'-phosphoadenylyl sulfate	mutant W53L, pH 7.4, temperature not specified in the publication	Bos taurus
0.00524	-	3'-phosphoadenylyl sulfate	mutant W53A, pH 7.4, temperature not specified in the publication	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P50227	isoform SULT1A1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylyl sulfate + 7-hydroxycoumarin	-	Bos taurus	adenosine 3',5'-bisphosphate + 7-hydroxycoumarin sulfate	-	?

Synonyms

Synonyms	Comment	Organism
SULT1A1	-	Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
42	45	30 min, 50% inactivation, wild-type and mutants	Bos taurus
45	-	melting temperature, wild-type peaks at 45°C, with a domain-suggestive shoulder at 49°C	Bos taurus
60	-	melting temperature, wild-type in presence of 3'-phosphoadenosine-5'-phosphate plus pentachlorophenol	Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.069	-	3'-phosphoadenylyl sulfate	mutant W53A, pH 7.4, temperature not specified in the publication	Bos taurus
0.125	-	3'-phosphoadenylyl sulfate	mutant W53L, pH 7.4, temperature not specified in the publication	Bos taurus
0.141	-	3'-phosphoadenylyl sulfate	wild-type, pH 7.4, temperature not specified in the publication	Bos taurus
0.18	-	3'-phosphoadenylyl sulfate	mutant W53Y, pH 7.4, temperature not specified in the publication	Bos taurus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
13	-	3'-phosphoadenylyl sulfate	mutant W53A, pH 7.4, temperature not specified in the publication	Bos taurus
24	-	3'-phosphoadenylyl sulfate	mutant W53L, pH 7.4, temperature not specified in the publication	Bos taurus
129	-	3'-phosphoadenylyl sulfate	mutant W53Y, pH 7.4, temperature not specified in the publication	Bos taurus
173	-	3'-phosphoadenylyl sulfate	wild-type, pH 7.4, temperature not specified in the publication	Bos taurus